Signalling

Part:BBa_K3651034

Designed by: Kayla Le   Group: iGEM20_Baltimore_BioCrew   (2020-10-03)


Constitutive expressor of Ferric Uptake Regulator Protein

J23100-FUR. This is a part that constitutively expresses Ferric Uptake Regulator Protein (FUR). In the presence of iron, iron-bound FUR can bind to a FUR box and block transcription. These FUR boxes are typically within promoters. We could use this part in conjunction with pfur1-mcherry (BBa_K3651026) to confer iron-sensitive expression of mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 183
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 104
    Illegal BsaI.rc site found at 320


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Categories
Parameters
None